gingseng (Panax ginseng) cells with the rolC gene from agrobacteria Agrobacterium rhizogenes can also cause the elevation of both the polyploidy and aneuploidy
نویسندگان
چکیده
The numbers of chromosomes and nucleoli in cultured cells of Vitis amurensis transformed with the rolB oncogene from A. rhizogenes have been studied. In general, the integration of the rolB gene in grape DNA mostly caused the elevation of the level of the chromosome variability, as well as higher numbers of nucleoli in the cultured cells. The possible influence of the observed chromosomal modifications on the pro ductivity parameters of the grape cell cultures is discussed. DOI: 10.1134/S1022795413060057 PLANT GENETICS
منابع مشابه
Mutation of Panax ginseng genes during long-term cultivation of ginseng cell cultures.
It has previously been shown that the nucleotide sequences of the Agrobacterium rhizogenes rolC locus and the selective marker nptII developed mutations during the long-term cultivation of transgenic cell cultures of Panax ginseng. In the present report, we analyzed the nucleotide sequences of selected plant gene families in the 20-year-old P. ginseng 1c cell culture and in leaves of cultivated...
متن کاملCell-autonomous behavior of the rolC gene of Agrobacterium rhizogenes during leaf development: a visual assay for transposon excision in transgenic plants.
We describe a genetic switch based on the Ac transposable element of maize and the rolC gene of Agrobacterium rhizogenes, a dominant gene, which has pleiotropic effects on plant growth and morphology. Moreover, rolC gene expression under the control of the 35S cauliflower mosaic virus promoter decreases chlorophyll content in transgenic tobacco plants. Chlorophyll is a visible cell-autonomous m...
متن کاملمطالعه اثر عصاره Panax ginseng بر تغییرات هیستومورفومتریک مخ و مخچه در نوزادان 14 روزه موش صحرایی مادران دیابتی
Introduction: Gestational diabetes induces developmental disorders in different parts of CNS and can change dimensions of the cerebrum, cerebellum and spinal cord. Different species of ginseng have been used for many years to control diabetes. This study investigated effect of extract of Panax ginseng on histomorphometric changes on cerebrum and cerebellum in 14 days offsprings rat from diabet...
متن کاملP 34: Evidence of Panax Ginseng in Multiple Sclerosis
Introduction: MS disease destroys the myelin of the central nervous system. MS causing damage to the myelin sheath of the brain and the spinal cord. The mechanism is hurt by the immune system .and disruption of myelin producing cells or disconnect interconnected processes by disconnect inner white. MS is an autoimmune disease when leukocyte antigen system varies autoimmune disease like MS ...
متن کاملIsolation and characterization of LHT-type plant amino acid transporter gene from Panax ginseng Meyer
A lysine histidine transporter (LHT) cDNA was isolated and characterized from the roots of Panax ginseng, designated PgLHT. The cDNA is 1,865 bp with an open reading frame that codes for a protein with 449 amino acids and a calculated molecular mass of 50.6 kDa with a predicted isoelectric point of 8.87. Hydropathy analysis shows that PgLHT is an integral membrane protein with 9 putative membra...
متن کامل